National point prevalence study on carriage of multidrug-resistant microorganisms in Dutch long-term care facilities in 2018

Abstract Objectives Long-term care facilities (LTCFs) may act as a reservoir of ESBL-producing Enterobacterales (ESBL-E) and carbapenemase-producing (CPE) for hospitals the general population. In this study, we estimated prevalence molecular epidemiology rectal carriage with ESBL-E CPE in residents Dutch LTCFs between March 2018 December 2018. Methods were geographically selected across country. For each LTCF, random sample tested To identify risk factors high and/or individual carriage, characteristics subset collected. WGS was conducted on isolates from an >10% all to institutional clonal transmission. Results A total 4420 159 included. The weighted mean 8.3% (95% CI: 6.8–10.0) no found. 53 (33%), where >10%, MLST using (wgMLST) performed. This included 264 isolates, majority being Escherichia coli (n = 224) followed by Klebsiella pneumoniae 30). Genetic clusters identified more than half (30/53; 57%) ESBL-positive LTCFs. Among E. blaCTX-M-15 (92/224; 41%) blaCTX-M-27 (40/224; 18%) most prevalent ESBL-encoding genes. K. common (23/30; 80%). Conclusions is resistance observed mainly predominance blaCTX-M-27. seems comparable previously reported